NATIVE ARBUSCULAR MYCORRHIZAL FUNGI IN THE AGROECOLOGICAL FARM OF UNIMINUTO, VILLAVICENCIO META

Abstract

In the soils of the Orinoquía there is a great diversity of microorganisms, such as the fungi that form arbuscular mycorrhizae-AMF, which, in addition to their presence, are relevant for the different interactions they generate and the ecosystem services they provide. For this reason, this research sought to characterize the populations of native AMF associated with two areas with different degrees of anthropic intervention in the Agroecological farm of UNIMINUTO, Villavicencio-Meta. For this, six rhizosphere soil samples were taken in each area and spore extraction was performed using the decanting and wet sieving technique, followed by centrifugation in a 70% sucrose gradient. The spores found were counted, selected and identified. A chemical analysis of the soils was carried out and the relationship of the parameters with the number of spores (Spearman Correlation) was determined. Likewise, a comparison of means (Mann Whitney U test) was carried out, finding statistically significant differences (N: 18, p value: 0.0001) between the density of spores present in the two study areas, with the forest being the place with the highest density. (7640.5 spores/100 g of soil) compared to the grassland area (3323.1). A correlation was found between the number of spores and each of the 18 parameters evaluated, except for pH. A total of 46 morphospecies of spores belonging to the genera Glomus (16), Acaulospora (12), Gigaspora (7), Scutellospora (4), Claroideoglomus (2), Sclerocystis (2) and Funneliformis (1) were identified. What was found is a contribution to the generation of solutions to face problems present in agroecosystems